Dermal Irritation Testing | Bioscience Labs

Dermal Irritation Testing

Dermal Irritation Testing

The EpiDerm™ Skin Model uses a culture of normal human-derived epidermal keratinocytes that forms a multi-layered model of the human epidermis. This model is mitotically and metabolically active and closely mimics the responses to products exhibited by its in vivo counterpart (RIPT). 

  • Dermal Irritation
  • Dermal Corrosion
  • Percutaneous Absorption
  • Mitotically and metabolically active and closely mimics the responses to products exhibited by its in vivo counterpart

Dermal irritation testing is conducted using the ET-50 method (time of exposure needed for a test article to reduce the viability to 50% of control tissues) and the EpiDerm tissue model.  This model consists of normal (non-cancerous), human cell-derived epidermal keratinocytes cultured to form a multilayered model of the human epidermidis.  The dermal irritation evaluation used at BioScience Laboratories involves applying neat test articles to the EpiDerm tissue model.  This test method closely approximates the responses to product exposure exhibited by its in-vivo counterpart, the Human Repeat-Insult Patch Testing.  Based on the ET-50 and comparison to benchmark materials, the degree of dermal irritancy can be estimated.  An additional benefit of dermal irritation testing is that, in many instances, results obtained using animal-based testing do not correlate well with the actual response seen in human tissue.


In-Vitro Testing Laboratory

Scientists in our In-Vitro testing labs are well trained on ASTM, AATCC, AOAC, CLSI and EN standard methods. The efficacy testing services of this laboratory include MIC/MBC, biofilm prevention and removal, time-kill kinetics, clean room disinfectant validation, zone of inhibition and custom efficacy studies for disinfectants, antimicrobials and other products requiring EPA registrations and FDA 510(k) submissions. Studies in our…

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OECD 431

In-Vitro Skin Corrosion: Human Skin Model Test

In this method, the test material (solid or liquid) is applied to a three-dimensional human skin model and corrosive materials are identified by their ability to produce a decrease in cell viability below defined threshold levels at specified exposure periods. The main principle…

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OECD 439

In-Vitro Skin Irritation: Reconstructed Human Epidermis Test Method

This method is based on reconstructed human epidermis, which closely mimics the properties of the upper layers of the human skin. Cell viability is measured by enzymatic conversion of the vital dye MTT into a blue formazan salt that is quantitatively…

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