Dermal OECD 439

Dermal OECD 439

In-Vitro Skin Irritation: Reconstructed Human Epidermis Test Method

This method is based on reconstructed human epidermis, which closely mimics the properties of the upper layers of the human skin. Cell viability is measured by enzymatic conversion of the vital dye MTT into a blue formazan salt that is quantitatively measured after extraction from tissues. Irritant test substances are identified by their ability to decrease cell viability below defined threshold levels. This procedure may be used to determine the skin irritancy of test substances as a stand-alone replacement test for in vivo skin irritation testing, or as a partial replacement test, within a tiered testing strategy.

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BioScience Laboratories assists clients with new product development, R&D projects, marketing claims, and supporting EPA, FDA, EU, and Health Canada label claims. Combining our virology capabilities with over 23 years of experience in conducting GLP and GCP compliant studies, we can offer a start-to-finish solution. Our virology laboratory personnel are well-trained on the…

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