In-Vitro Laboratory

In-Vitro Laboratory

Scientists in our In-Vitro testing labs are well trained on ASTM, AATCC, AOAC, CLSI, and EN standard methods. The efficacy testing services of this laboratory include MIC/MBC, biofilm prevention and removal, time-kill kinetics, clean room disinfectant validation, zone of inhibition and custom efficacy studies for disinfectants, antimicrobials and other products requiring EPA registrations and FDA 510(k) submissions. Studies in our in-vitro lab can also be designed and executed to differentiate existing products within the market and support unique label claims.

BioScience provides a wide scope of in-vitro test evaluation possibilities from one to two microorganism screening evaluations to evaluations using more than 1,500 ATCC strains and clinical isolates. 

Our In-Vitro Laboratory uses tissue models prepared by the company leading the development of alternatives to animal testing methods to perform safety testing on cosmetics, surfacant-based products, pharmaecuticals and chemicals as well as products for personal care, hair care, and household cleaning . These tests include Ocular Irritation, Skin Irritation, Dermal Corrosion, Oral Irritation, and Cytotoxicity. 

Services Include:
  • Time-Kill Kinetic/ Suspension Test
  • MIC/MBC Testing
  • Antimicrobial Effectiveness Testing
  • BioFilm Prevention & Removal
  • Zone-Of-Inhibition
  • Disinfectant Testing
  • Medical Devices Testing
  • Testing of Bound Antimicrobials
  • Safety Testing
Methods
Services

PCPC 1

Preservation Testing of Water-Miscible Products Challenge testing a formula with known microorganisms to verify that if the products become contaminated, the preservative system is sufficient to…
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AOAC 955.17 FUNGICIDAL ACTIVITY OF DISINFECTANTS; USING TRICHOPHYTON MENTAGROPHYTES

This standard test method recommended by the EPA provides efficacy data for Test Products designed as disinfectants with antifungal applications.  The method uses T. mentagrophytes…
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AOAC 966.04 SPORICIDAL ACTIVITY OF DISINFECTANTS

This standard method tests the efficacy of disinfectants against spore-forming bacteria on hard surface carriers.  This standard test is suitable for determining sporicidal activity of…
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AOAC 965.13 DISINFECTANTS (WATER) FOR SWIMMING POOLS

This standard test method recommended by the EPA provides efficacy data for Test Products designed to disinfect spa and pool water.  BioScience Labs offers this…
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ISO 10993-5

Biological Evaluation of Medical Devices This test method describes methods to assess the in-vitro cytotoxicity of medical devices. These methods specify the incubation of cultured…
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ASTM E2871-13

Single Tube Method BioScience Labs provides services for evaluation of product efficacy in the prevention and/or removal of biofilms using in-vitro techniques. This method is typically…
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ASTM - E640

Standard Test Method for Preservatives in Water-Containing Cosmetics This test method covers the determination of the suitability of preservatives for use in cosmetic formulations. It…
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ASTM - E2362

Standard Practice for Evaluation of Pre-saturated or Impregnated Towelettes for Hard Surface Disinfection This test method may be used to determine if a pre-saturated or impregnated…
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AOAC Germicidal Towelette Test

This standard method evaluates the efficacy of towelette disinfectants according to ASTM E2362-09 based on EPA SOP’s.  BioScience Labs offers this service to provide the…
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EN 1040

Chemical Disinfectants and Antiseptics- Quantitative Suspension Test for the Evaluation of Basic Bactericidal Activity of Chemical Disinfectants and Antiseptics This European Standard applies to active…
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ASTM E2647-08

Drip Flow Reactor Protocol BioScience Labs provides services for evaluation of product efficacy in the prevention and/or removal of biofilms using in-vitro techniques. This method represents…
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USP 51

ANTIMICROBIAL PRESERVATIVE EFFECTIVENESS TEST (PET) The U.S. Pharmacopeia describes in General Chapter 51, the assay they recommend for testing preservative efficacy. The USP divides products…
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AOAC 961.02 GERMICIDAL SPRAY PRODUCTS AS DISINFECTANTS

This standard test method recommended by the EPA provides efficacy data for Test Products designed as germicidal spray products.  The method uses three standard bacterial…
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ASTM - E2197

Standard Quantitative Disk Carrier Test Method for Determining Bactericidal, Virucidal, Fungicidal, Mycobactericidal, and Sporicidal Activities of Chemicals This test method is designed to evaluate the…
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EN 1275

Quantitative suspension Test for the Evaluation of Basic Fungicidal or Basic Yeasticidal Activity of Chemical Disinfectants and Antiseptics This European Standard applies to active substances…
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ASTM- E1054

Standard Test Method for Evaluation of Inactivators of Antimicrobial Agents The effectiveness of antimicrobial agents incorporated into disinfectants, sanitizers, and antiseptics is measured by their…
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ASTM - E2280

Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agent(s) in Polymeric or Hydrophobic Materials This test method is designed to evaluate (quantitatively) the…
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ASTM - E2149

Standard Test Method for Determining the Antimicrobial Activity of Antimicrobial Agents Under Dynamic Contact Conditions This test method is designed to evaluate the antimicrobial activity…
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EN 14348

Quantitative Suspension Test for the Evaluation of Mycobactericidal Activity of Chemical Disinfectants in the Medical Area Including Instrument Disinfectants  This document specifies a test method…
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ASTM - E2783

Standard Test Method for Assessment of Antimicrobial Activity for Water-Miscible Compounds Using a Time-Kill Procedure This test method measures the changes of a population of…
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AATCC - 100

Antimicrobial Finishes on Textile Materials This test method provides a quantitative procedure for the evaluation of the degree of antibacterial activity. Assessment of antibacterial finishes…
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AOAC 965.12 TUBERCULOCIDAL ACTIVITY OF DISINFECTANTS

This standard test method recommended by the EPA demonstrates efficacy on hard non-porous surfaces against M. bovis.  BioScience Labs offers this service to provide the…
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ASTM- E1153

Standard Test Method for Efficacy of Sanitizers Recommended for Inanimate Non-Food Contact Surfaces This test method is used to evaluate the antimicrobial efficacy of sanitizers…
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EN1276

Quantitative Suspension Test for the Evaluation of Bactericidal Activity of Chemical Disinfectants and Antiseptics used in Food, Industrial, Domestic and Institutional Areas This European Standard…
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EN 13727

Quantitative Suspension Test for the Evaluation of Bactericidal Activity in the Medical Area This European Standard applies to products that are used in the medical…
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ISO 11930

The International Organization for Harmonization is designed for cosmetic formulations. Unlike the USP 51 or EP 5.1.3, ISO does not divide products into different categories.…
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AOAC 955.16 CHLORINE (AVAILABLE) IN DISINFECTANTS; GERMICIDAL EQUIVALENT CONCENTRATION

This standard test method provides efficacy data that demonstrates equivalent performance of Test Product equivalent concentrations to sodium hypochlorite.  This study evaluates sanitizing rinses for…
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EN 13624

Quantitative Suspension Test for the Evaluation of Fungicidal Activity of Chemical Disinfectants for Instruments used in the Medical Area This European Standard applies to products…
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AOAC 955.14, 955.15, and 964.02 Use Dilution Method

These standard methods test the efficacy of disinfectants against three standard bacteria on hard surfaces.  These standard tests are used for products in the EPA…
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EN 13697

Quantitative Non-Porous Surface Test for the Evaluation of Bactericidal and/or Fungicidal Activity of Chemical Disinfectants Used in Food, Industrial, Domestic and Institutional Areas This method…
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OECD 437

Bovine Corneal Opacity and Permeability (BCOP) Test Method for Identifying Ocular Corrosives/Severe Irritants and Substances not Classified as Irritants The Bovine Corneal Opacity and Permeability…
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CLSI M07-A10

Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically Susceptibility testing is indicated for any organism that contributes to an infectious process warranting…
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ASTM E2799-12

MBEC Reactor Protocol BioScience Labs provides services for evaluation of product efficacy in the prevention and/or removal of biofilms using in-vitro techniques.  This method serves…
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AOAC 960.09 GERMICIDAL AND DETERGENT SANITIZING ACTION OF DISINFECTANTS TEST

This standard test method tests the efficacy of food contact sanitizers against S. aureus and E. coli.  These studies are used for products in the…
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EN1650

Quantitative Suspension Test for the Evaluation of Fungicidal activity of Chemical Disinfectants and Antiseptics Used in Food, Industrial, Domestic and Institutional Areas This method describes…
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ASTM E2562-12

CDC Biofilm Reactor Protocol BioScience Labs provides services for evaluation of product efficacy in the prevention and/or removal of biofilms using in-vitro techniques.  This method…
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ASTM - E2406

Standard Test Method for Evaluation of Laundry Sanitizers and Disinfectants for Use in High Efficiency Washing Operations This test method is designed to evaluate sanitizing/…
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JIS L1902 Determination of Antibacterial Activity of Antibacterial Materials

This standard test method evaluates the antibacterial activity of antibacterial finished textile products including woven and non-woven material.  The materials are inoculated with a known…
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ASTM - E2274

Standard Test Method for Evaluation of Laundry Sanitizers and Disinfectants This test method is designed to evaluate sanitizing/ disinfectant laundry detergents/additives for use in top-loading…
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Minimum Inhibitory Concentration (MIC) Test

Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) testing define a test material's potency in terms of the concentration at which it will inhibit…
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First Aid Wound Management

In-vitro evaluations as described under II-A, and as follows. Screening zone-of-inhibition studies. Studies per methodology prescribed by the FDA: 21 CFR Sections 333 and 369,…
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Minimum Bactericidal Concentration (MBC) Test

The MBC testing is designed to find the lowest possible concentration at which an antimicrobial agent will kill a designated microorganism.  The MBC is performed after a Minimum…
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Disinfectant and Sanitizer Testing Services

Bioscience Labs performs these challenging antimicrobial efficacy studies.  Whether you need the AOAC Germicidal Spray Products as Disinfectants Test or the AOAC Use-Dilution Test (for disinfectants requiring dilution) we…
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Antimicrobial Preservative Efficacy Testing

Antimicrobial Effectiveness Tests, or Preservative Effectiveness Tests, are performed to determine the efficacy of the antimicrobial preservatives added to many pharmaceutical and cosmetic products to…
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Bandage Material Physical Properties

The Materials Testing Lab features temperature and humidity control along with the ability to test for the following product characteristics: Tensile Strength Peel Adhesion Our…
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Zone of Inhibition

This diffusion test is used to evaluate the -static/-cidal activity of water-soluble, leachable antimicrobial formulations that will diffuse into an nutrient agar medium.  Selected challenge species are…
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Clean Room Disinfectant Validation

BSLI also performs cleaning validation studies for which AOAC-like methods are designed to appraise the ability of disinfectant products used in specific cleaning routines to eliminate bacteria…
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Bovine Corneal Opacity and Permeability (BCOP)

The Bovine Corneal Opacity and Permeability (BCOP) test is an in vitro method to evaluate chemicals inducing serious eye damage or serious eye irritation.  This…
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Time-Kill Kinetics - Antimicrobial Efficacy Test

Time-Kill In Vitro assay tests are referred to as “suspension tests” because a measured volume of the suspension of challenge bacteria or fungi is transferred into a…
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Wound Healing Properties of Product Materials

Evaluations of rate of cell proliferation, measured as a function of cellular protein expression (e.g., growth factors) in a 3-D model of wound re-epitheliazation.
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Bandage and Wound Care Absorbency Testing

We possess the equipment and facilities to evaluate the absorbency and vapor permeablitiy of bandage and wound care dressings. 
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General Irritation Testing

The Skin Technology Center performs studies of the irritancy potential of topical antimicrobial products by means of multiple applications (15 or more) of a product…
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Agar Patch - Antimicrobial Persistence Test

The FDA does not have a process in place for formal evaluation of results of such studies and has no recognized official method for demonstrating persistence,…
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In-Vitro Wound Management Evaluations

In-vitro evaluations of antimicrobial properties versus ATCC or clinically isolated strains of bacteria relevant to surgical-, burn-, and catheter-related infections. Time-Kill Kinetic studies (planktonic and…
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Injection Site Protection

Injection sites are a common source of nosocomial infections. Proper pre-treatment and prophylaxis of these sites is important to reducing healthcare costs and improving patient well-being.…
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