Bovine Corneal Opacity and Permeability Test - BCOP Test

BCOP testing may be used as a tiered testing approach, in conjunction with the EpiOcular^TM Method. The EpiOcular^TM Method uses an in vitro model of the human corneal epithelium and allows differentiation of moderate and mild irritants.  Recently, in conversations with the EPA, BioScience has been encouraged to learn that the EPA considers the BCOP test and the EpiOcular^TM methods the most valid in vitro alternatives to the Rabbit Draize eye irritancy test.

The Bovine Corneal Opacity and Permeability (BCOP) is an in vitro test method to evaluate ocular irritation and corrosivity as an alternative to traditional animal testing methods. The BCOP test can be used to classify substances as ocular corrosives and severe irritants, as defined by the EPA, EU, and the GHS. Substances can be classified without further testing in rabbits (Draize test).  An in vitro testing strategy for eye irritation is recommended in OECD 405, Guideline for Acute Eye Irritation/Corrosion.  OECD TG 437, Guideline for the BCOP test, describes the procedures used to evaluate the potential corrositivity or severe irritancy in isolated bovine corneas; the endpoints measured are decreased light transmission (opacity) and increased passage of sodium fluorescein dye (permeability).  The values  of the two evaluations are summed to derive an in vitro irritancy score (IVIS), which is then used to classify the irritancy of the substance.  An optional  histopathological evaluation can be performed to further characterize the toxic effect of the substance.

For further information on BCOP testing please click HERE